Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and osmads58-/- Panicles Transcriptomes

Purpose: The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Conclusions: Our study represents the detailed analysis of panicles transcriptomes, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Overall design: 1cm long panicles mRNA profiles of wild type (WT) and osmads58-/-