Single-cell transcriptomics of Cxcl13+ LN stromal cells. Single-cell transcriptomics of Cxcl13+ LN stromal cells

Purpose: The goal of this study is to characterise the follicular dendritic cells (FDCs) upon antigen-immunocomplex immunisation. Methods: FACS sorting of Live/Death- Podoplanin+ CD31- CD45- CXCL13-TdTomato+ after LN disgregation of CXCL13-CreTdTomato mice. Mice has been previously immunised with antigen-Ics at different time-points. Cells were loaded into 10x Genomics Chromium Platform, and sequenced using Illumina HiSeq 4000. Conclusions: Our study detects previously identified stromal cell populations and shows the heterogeneity present on the FDCs. We detect 3 different FDC clusters. Overall design: FACS-sorted CXCL13-TdTomato+ stromal cells were used for single-cell RNA-sequencing (10x Genomics Chromium Platform), and sequenced using Illumina HiSeq 4000.