Best fit parameters for ITC measurements of IlsA binding to ferritinsa.

aThe reported thermodynamic quantities are apparent values and include the contributions to the overall equilibrium from ferritin and buffer species in different states of protonation.Standard errors from replicate determinations are indicated.bCalculated from ΔG0 = −RTlnK.cCalculated from ΔS0 = (ΔH°−ΔG°)/T.HuHF, recombinant human H-chain ferritin; HuLF, recombinant human L-chain ferritin; HuH/LF, recombinant heteropolymer ferritin of 21H-chains and 4L-chains; MoHF, recombinant mouse H-chain ferritin. All experiments were repeated two to four times.