mRNA profiling upon knockdown of circRNA-HIPK2

Despite advances in drug development, global mortality rates following heart failure (HF) remain still high, underscoring the critical need for novel therapeutic strategies. Emerging evidence highlights cardiac macrophages as promising cellular targets for intervention. In our investigation, we identified a circular RNA-HIPK2 (circHIPK2.) exhibiting close association with macrophage polarization dynamics following myocardial infarction (MI). To elucidate its functional significance and associated transcriptomic alterations, we performed targeted knockdown of circHIPK2. in macrophage cells through siRNA transfection, comparing experimental groups (circHIPK2 siR) with controls (ctrl siR). Overall design: Expression profiling by high throughput sequencing on Raw264.7 macrophages upon transfection with 100 nM circRNA-HIPK2 siRNA and scramble siRNA, followed by LPS (100nM/mL) stimulation. Total RNA was harvested, sequencing was performed in triplecates.