Proteins identified by LC-MS/MS in GCAP2 immunoprecipitation experiments.

The table lists proteins identified in GCAP2 immunoprecipitation assays from retinal homogenates of GCAPs−/− bGCAP2 line E, GCAPs−/− bEF−GCAP2 line B and GCAPs−/− mice (ctrl). Data is shown from two independent experiments (three columns per experiment). Last six columns indicate the number of peptides identified for each protein in each sample, indicative of the relative levels of coimmunoprecipitated proteins. GCAP2 was immunoprecipitated to similar levels in control line E and mutant line B, but 14-3-3 isoforms coimmunoprecipitation with GCAP2 occurred substantially more efficiently in mutant line B than in control line E, indicating 14-3-3 selective binding to GCAP2 locked in its Ca2+-free form. GCAP2, guanylate cyclase activating protein 2.