H3K9ac ChIP-seq in WT, SAGA and ATAC mutant mouse embryonic stem cells
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https://www.ncbi.nlm.nih.gov/sra/SRP322141
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资源简介：
Assess H3K9ac levels in WT and mutant ES E14 strains using ChIP-seq. Mutant strains are depleted for subunits of the coactivator complexes SAGA (Supt7l KO) or ATAC (AID-Yeats2) or of HAT subunits (AID-Tada3, Tada2a+Tada2b KO) using constitutive knock-out (KO) or the auxin-inducible degron (AID) system. Overall design: ChIP-seq was performed with 49 ug of mouse chromatin and 1 ug of Drosophila S2 cell spike-in chromatin with 2 ug of H3K9ac antibody (Abcam, Cat#ab4441) in one replicate.
创建时间：
2021-09-16
