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Data from: Single cell RNA-seq data of E18 fetal thymocytes from HEB Vav-iCre and Id3-KO mice and their wild type littermate counterparts

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DataCite Commons2026-04-27 更新2026-04-25 收录
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https://datadryad.org/dataset/doi:10.5061/dryad.08kprr5cq
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γδ T cells that produce IL-17 (γδT17) play essential roles in barrier immunity and autoimmunity, but the gene networks that install their functions are not well understood. To understand how HEB and Id3 regulate γδT17 cell development, we conducted single-cell RNA-sequencing on fetal thymic γδ T cells from Tcf12-deficient (HEB cKO) and Id3 knockout (Id3-KO) E18 embryos. Four datasets were generated. The first two consist of WT and HEB cKO datasets derived from sorted gamma-delta T cells. The second two consist of WT and Id3-KO datasets derived from magnetically enriched for CD4/CD8-negative cells. HEB datasets were generated with 10X Genomics 5' chemistry, and Id3 datasets were generated with 10X Genomics 3' chemistry. Raw sequence files were processed using Cell Ranger to generate matrix files, which have been uploaded here. We also include R-markdown files (RMD) and R-markdown HTML output files to provide code and programs used for the data analysis shown in the associated paper.
提供机构:
Dryad
创建时间:
2025-12-08
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