Differences in proteasome targeting in Arabidopsis leaf mesophyll, epidermal and vascular cells

Data from ProteomeXchange, PXD ID: PXD000942. File: 20130401_94_Vasc_UBA2_2_1ex.mgf. From ProteomeXchange: {{i}} For the detailed and sensitive detection of the responses to a specific treatment it is important to perform tissue or cell type specific analyses, but this is not easily achievable for the different leaf tissues. Here we developed a method termed MeSelect to effectively separate leaf epidermis, vascular and mesophyll cells basically without contamination from other tissues. The high yield of the MeSelect method allowed for tissue specific high-throughput proteome analyses after inhibition of the proteasome with Syringolin A and the affinity enrichment of polyubiquitylated proteins in epidermis, mesophyll and vasculature. {{/i}}