Single cell RNA-sequencing on stimiluated mouse lung lipofibroblast-like cells and control cells.

To achieve the in vitro functions of lipofibroblasts, isolated murine lung fibroblasts were cultured and biochemically stimulated. Stimulated fibroblasts in which a lipofibroblast-like phenotype had been induced using conventional methods displayed pronounced lipid inclusions. scRNA-seq analysis of lipofibroblast-like cells demonstrated that stimulated lipofibroblast-like cells displayed high transcript expression of canonical makers, like Plin2, as reported by others.