Skin, gut, and sand metagenomic data tables on placebo-controlled sandbox biodiversity intervention study

The metagenomic data are related to the published research of “A Placebo-controlled double-blinded test of the biodiversity hypothesis of immune-mediated diseases: Environmental microbial diversity elicits changes in cytokines and increase in T regulatory cells in young children” and Data in Brief article "Skin, gut, and sand metagenomic data on placebo-controlled sandbox biodiversity intervention study".
This database contains 16S ribosomal RNA (rRNA) metagenomics of sandbox sand and skin and gut microbiota of children in the intervention and placebo daycares. In intervention daycares, children aged 3–5 years were exposed to playground sand enriched with microbially diverse soil. In placebo daycares, children were exposed to visually similar as in intervention daycares, but microbially poor sand colored with peat. Sand, skin and gut metagenomics were analyzed at baseline and after 14 and 28 days of intervention by high throughput sequencing of bacterial 16S rRNA gene on the Illumina MiSeq platform.
We herein report the richness, diversity, relative abundance (Table 1) and community composition (Table 2) of sand bacteria between enriched intervention and placebo sandbox sand, and bacterial relative abundance shifts on the skin (Table 3) and in the gut between intervention and placebo groups (Table 4 and 5).

该元基因组数据与已发表的《关于免疫介导性疾病多样性假设的安慰剂对照双盲试验：“环境微生物多样性引起幼儿细胞因子变化和调节性T细胞增加”》及《安慰剂对照沙盒生物多样性干预研究皮肤、肠道和沙子元基因组数据》两篇研究文章相关。本数据库包含干预组和安慰剂组托儿所中儿童沙子、皮肤和肠道微生物群落的16S核糖体RNA（rRNA）元基因组学数据。在干预组托儿所中，3至5岁的儿童接触了富含微生物多样性土壤的游乐场沙子。在安慰剂组托儿所中，儿童接触了与干预组托儿所相似的视觉沙子，但微生物含量较低，并用泥炭染色。沙子、皮肤和肠道元基因组学分析在基线以及干预后14天和28天，通过Illumina MiSeq平台对细菌16S rRNA基因进行高通量测序。本研究报告了富集干预组和安慰剂组沙盒沙子中细菌的丰富度、多样性、相对丰度（表1）和群落组成（表2），以及干预组和安慰剂组之间皮肤（表3）和肠道（表4和表5）中细菌相对丰度的变化。