Bulk Segregant Analysis of copepod metamorphosis rate

This study aimed to identify genomic loci associated with metamorphosis rate in the copepod Tigriopus californicus. F1 hybrids were generated between two divergent populations: SD (from San Diego, CA), and SH (from Strawberry Hill, OR). Once F1 female developed egg sacs, the F2 larvae from each reciprocal cross (SDfemale x SHmale, and SHfemale x SDmale) were hatched in large plates and monitored daily. When each individual reached metamorphosis into a copepodid juvenile, the individual was scored for the date and frozen. After ~3000 individuals were scored for each cross, the 10% slowest and the 10% fastest were each pooled for library prep and sequencing.