Achilles_v3.3.7_README.txt

GeCKOv2 Achilles dataset33 cell lines<br>Achilles_v3.3.7_lognorm.gct contains the replicate-level read counts per million<br>Achilles_v3.3.7a.gct is the guide-level log2FC data used for analyses in the publication. It is not normalized across cell lines, but the median of the negative control sgRNAs are subtracted in each sample (i.e. a score of 0 represents median of the negative controls).<br>Achilles_v3.3.7_gene-soln.gct is the version of the data used for gene-level analyses across cell lines in the publication. Samples are z-score normalized and sgRNAs with &gt;1 perfect match are removed.<br>Achilles_v3.3.7_ABSOLUTE_CN_segtab.txt contains the ABSOLUTE copy number segments for each of the 33 samples.<br>Achilles_v3.3.7_sgRNA_mappings.txt contains the mapped genomic locus (loci) for each sgRNA.<br><br><br>Analysis steps v3.3.7a:<br> 1. QC and identify reps with reproducibility &lt;0.8 or are outliers on PCA or fail FP 2. remove sgRNAs(guides) with low counts in DNA samples: completely remove shRNAs with median &lt;=1 3. remove replicates that fail QC measures 4. fold change(FC) with DNA pool samples 5. zero-center median of negative controls 6. collapse replicates per line 7. sgRNAs are mapped to genes using(Achilles_v3.3.7a.gct)<br>Analysis steps v3.3.7:<br> 1. QC and identify reps with reproducibility &lt;0.8 or are outliers on PCA or fail FP 2. remove sgRNAs(guides) with low counts in DNA samples: completely remove shRNAs with median &lt;=1 3. remove sgRNAs(guides) that have &gt;1 perfect match anywhere in the reference genome 4. remove replicates that fail QC measures 5. fold change(FC) with DNA pool samples 6. Z score normalize each cell line 7. collapse replicates per line 8. sgRNAs are mapped to genes (Achilles_v3.3.7.gct) 9. ATARiS (pval=0.05) was run on guide-level data (Achilles_v3.3.7_gene-soln.gct)<br><br>