RNA-seq of mouse pancreatic pericytes from wild-type and Tcf7l2-mutated mice

To characterise their gene expression, wild type pancreatic pericytes were isolated based on the YFP-labeling from Nkx3.2-Cre;EYFP mice. As control, whole isolated islets were analysed.To identify Tcf7l2-depenedent genes, pancreatic pericytes were isolated from Nkx3.2-Cre;EYFP;Tcf7l2 flox/flox transgenic mice, and compared to wild type cells.为表征其基因表达谱，研究人员基于黄色荧光蛋白（YFP）标记从Nkx3.2-Cre;EYFP小鼠中分离野生型胰腺周细胞；作为对照，同时对分离的完整胰岛进行分析。为鉴定Tcf7l2依赖型基因，研究人员从Nkx3.2-Cre;EYFP;Tcf7l2 flox/flox转基因小鼠中分离胰腺周细胞，并将其与野生型细胞进行比较。