Transcriptional changes following HIV infection of choroid plexus brain organoids

We employed iPSC-derived choroid plexus brain organoids to explore the impact of HIV infection on microglia, elucidating alterations in the host gene profile within the central nervous system. We conducted single-cell RNA sequencing to elucidate the changes in microglia upon HIV infection, leading to neural cell damage Overall design: Choroid plexus brain organoids were infected with mock medium or HIV virus. We pooled 8 or more brain organoids and performed 10x single-cell RNA sequencing. To investigate changes in gene expression in microglia upon HIV infection, we enriched these cells using CD11B+ microbeads