Systematic mapping of 3D enhancer interactions reveals lineage specific regulatory networks during early mouse developmental decisions [ChIP-Exo]

To model and characterize the chromatin regulatory landscape of the very first developmental cell fates, we made use of three previously derived and well-characterized Trophoblast Stem Cell (TSCs), Embryonic Stem Cell (ESC) and Extra Embryonic Stem Cell lines (XENs) We performed ChIP-Exo for CTCF to map the insulator regions in ESC, TSC and XEN Characterization of binding sites of CTCF with ChiP-Exo for all three cell types.