Data from: Detection of the 1016Gly and 989Pro knockdown resistance mutations in Florida, USA Aedes aegypti

Aedes aegypti is a major arboviral disease vector and is therefore a critical target for control by public health programs. Early eradication efforts have shown that Ae. aegypti can rapidly build insecticide resistance and now, resistance to pyrethroids, the major class of adulticides used for operational control, is common in many populations. A major contributor to this resistance is a group of knockdown resistance (kdr) SNPs that normally exist in distinct ensembles in the Western Hemisphere and the Indopacific with little known mixing. In this study, we detected, and confirmed using multiple methods, two Indopacific kdr SNPs, V1016G and S989P, in three recent collections from Osceola County, Florida. This represents a large expansion of the geographic range of the Indopacific kdr SNPs. We consider the implications of this finding on future insecticide resistance surveillance studies, including assessing the ability of our current screening tools to detect these SNPs. We find that the presence of the Indopacific SNPs requires modification of existing resistance screening protocols and requires further work to understand the operational implications for mosquito control. The information in this repository consists of the data supporting the original detection and the confirmation of the presence of the Indopacific mutations. It also includes sequencing protocols, analysis scripts, and sequencing metadata used in the study.

埃及伊蚊作为重要的虫媒病毒传播媒介，对公共卫生项目而言，是其控制策略中的关键靶点。早期的根除努力表明，埃及伊蚊能够迅速产生对杀虫剂的抗性。如今，对拟除虫菊酯类化合物（成人杀虫剂的主要类别，用于操作控制）的抗性在许多种群中已成为常态。此类抗性的主要诱因是一组敲倒抗性（kdr）单核苷酸多态性（SNPs），这些SNPs通常在美洲和印度-太平洋地区独立存在，相互混合的情况鲜为人知。在本研究中，我们通过多种方法检测并确认了两种印度-太平洋地区的kdr SNPs，即V1016G和S989P，它们存在于佛罗里达州奥西奥拉县的三份近期收集样本中。这一发现标志着印度-太平洋地区kdr SNPs地理分布范围的显著扩张。我们考虑了这一发现对未来杀虫剂抗性监测研究的影响，包括评估我们当前筛选工具检测这些SNPs的能力。我们发现，印度-太平洋SNPs的存在要求对现有的抗性筛选方案进行修改，并需要进一步的研究以理解其对蚊子控制操作层面的影响。本仓库中的信息包括支持原始检测和确认印度-太平洋突变存在的数据、测序方案、分析脚本以及研究中使用的测序元数据。