Figure S1 - IL-2 Mediates CD4⁺ T Cell Help in the Breakdown of Memory-Like CD8⁺ T Cell Tolerance under Lymphopenic Conditions

IL-2 neutralization depletes Tregs in lymphopenic InsHA mice. Mice described in Figure 3 were sacrificed at day 13 after transfer and expression of CD25, and Foxp3 was assessed on gated endogenous CD4+ Thy1.1− T cells in LN. The mean percentages (± SD, n = 3) of CD4+ CD25+ FoxP3+ T cells are indicated. Data from one representative experiment out of four, including an average of three mice per group, are presented. A 30% reduction in the frequency of Tregs was observed in InsHA mice treated with anti-IL-2 mAbs as compared to isotype control treated mice. (0.70 MB EPS)