Effect of splenectomy on liver regeneration after 70% resection in a mouse model.

In this work, the effect of splenectomy on liver regeneration was studied. For this purpose in the animals were reproduced splenectomy, and after 7 days 70% liver resection was performed. Animals in which the spleen was intact and only 70% liver resection was performed served as a comparison group. Animals in which only the spleen was removed also served as controls. The animals were removed from the experiment after 24 hours, 3 and 7 days. Macrophages were isolated from the livers of control animals as well as from the liver after 70% resection by magnetic sorting using the F4/80 marker. RNA was isolated from the obtained macrophages and used for transcriptome analysis. Macrophages in the regenerating liver were transcriptomically enriched for signaling pathways associated with monocyte migration, cell adhesion and cell death. In the first series of experiments, liver resection was performed in intact animals (n=25); in the second series of experiments the animals were splenectomized 7 days before the resection (n=25). Intact animals (n=6) and splenectomized animals with preserved liver (n=6) were used as respective controls. The animals were withdrawn from the experiment 24 hours, 72 hours and 7 days post-resection using a CO2 chamber. Macrophages were isolated from the liver using magnetic sorting for F4/80 and their gene expression profiles were analyzed using Clariom™ S Assay, mouse.