Genome-wide transcriptomic analysis of Arabdiopsis mutants controling nuclear size in comparison to the wildtype. Genome-wide transcriptomic analysis of Arabdiopsis mutants controling nuclear size in comparison to the wildtype

We report genome-wide transcriptomic profile of 2 week-old Arabidopsis seedlings from pwo1 and crwn1 crwn2 mutants in comparison to the wildtype (Col-0) Overall design: Arabidopsis thaliana seedlings of Col-0, pwo1 and crwn1 crwn2 were grown vertically on MS plates in long-day conditions for 2 weeks. Afterwards, RNA from 3 biological replicates per genotype was isolated using RNeasy Plant Mini Kit (Qiagen) and NGS library created using TruSeq RNA Library Prep Kit v2 (Illumina). Paired-end sequencing was performed on HiSeq 2500 platform (Illumina).