RNA-seq of cultured cells derived from Parkinson's disease patients [PolyA RNA-seq]

High throughput sequencing of poly-A RNA from control- and sporadic Parkinson´s disease patient derived fibroblasts (n=15), induced pluripotent stem cells (n=31) and differentiated midbrain neurons (n=15). Fibroblasts and iPSCs do not show major differences on single gene level. In contrast, midbrain neurons derived from Parkinson´s disease patients show changes known to be associated with neurodegenerative diseases. Two-condition experiment in three cell types: Control- and Parkinson´s disease patient derived fibroblasts, induced pluripotent stem cells and differentiated midbrain neurons were compared. Fibroblast cell cultures were established from skin biopsies from six control patients and nine sporadic Parkinson´s disease patients and reprogrammed with the OSKM cocktail. Poly-A RNA libraries were prepared with the Illumina TruSeq® RNA Sample Preparation Kit v2 and sequencing was performed on an Illumina HiSeq1000 instrument (1x50 bp). Differential expression analysis was performed comparing control- and Parkinson´s disease patient derived cells within each cell type (fibroblasts, induced pluripotent stem cells or neurons) Please note that Raw data is only available via dbGAP due to privacy concerns