Macrophage deletion of Noc4l triggers endosomal TLR4/TRIF signal and leads to insulin resistance

To study the role of noc4l in macrophages in inflammation induced insulin resistance, BMDM cells were isolated from Noc4l wild type or Noc4l knock out mouse and then treated with or without LPS. By using microarray expression profiling with RNA extrated from BMDMs, we found Noc4l was related to inflammation. BMDM cells were isolated from Noc4l wild type or Noc4l knock out mice and treated with or without 100ng/ml LPS for 16h. Total RNAs of BMDMs were isolated and purified, and hybridized to the SurePrint G3 Mouse GE 8x60K microarray (Agilent). Microarray slides were washed and scanned with a DNA Microarray Scanner (Agilent), according to the standard protocol of the manufacturer.