Fig5E_RACE_upstream-primer_R2_LG316_annotated.tif

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with 99bp inserted sequences from the STOML2 or YKT6 gene. Unlike other experiments in this collection, the insertion in this case was inside the intron of the luciferase reporter. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences. In this case the position of the primer was such that the RACE would only pick up, if present, a 200 bp fragment originating from the inserted sequence.