Wound healing and anti-oxidant activities induced by Dersani Hydrogel with Alginate and Dersani Hydrogel ointments

Explore the role of these hydrogels in wound healing, this study assessed the effects of both, Dersani Hydrogel with Alginate (DHA) and Dersani Hydrogel (DH), in human skin keratinocytes and fibroblasts gene expression profiles in a wound healing context. Sodium alginate (SA) and culture medium were also included as controls. 1x106 HaCaT-SF or HDFn cells were seeded in 6 well plates and incubated at 37 °C until 70-80% confluence was reached. Then a gap (wound) was created by scratching the monolayer with a sterile 200 µl tip, then the cells were washed two times with phosphate buffered saline (PBS) 1X and incubated with the compounds DHA, DH and SA (0.625% w/v) dissolved in culture media containing 0.01% v/v Tween 20 during 12 h. Biological triplicates were analyzed.