Supplementary Table 1: Urine-based regenerative RNA biomarkers for urinary bladder wound healing

<b>Supplement Table 1.</b>
The expression of genes in morning and evening<br>

<b>Abstract</b>

<b>Background:</b> This study aimed to investigate the expression of regeneration-related
genes in canine urine during bladder repair. <b>Materials &amp; methods:</b>
Canine urine samples were collected after partial cystectomy. Regenerative mRNA
of hypoxia-inducible factor (HIF), vascular endothelial growth factor (VEGF),
key stem cell transcription factors, and cholinergic signals were detected. <b>Results:</b>
HIF-1α, VEGF, CD44, interleukin-6, and prominin 1 expression in canine urine
after partial cystectomy exhibited two similar peaks at ~2 weeks. HIF-1α and
VEGF expression was higher in the afternoon than in the morning. The expression
of key stem cell transcription factors and cholinergic signals also exhibited a
rhythm along with bladder healing. <b>Conclusions:</b> The expression of
HIF-1α, VEGF, key stem cell transcription factors, and cholinergic signals
exhibited a time curve distribution during canine bladder healing. The
expression trend of some regenerative genes was similar during bladder healing,
and a cooperative effect may exist.