Supplementary Table 1: Urine-based regenerative RNA biomarkers for urinary bladder wound healing

<b>Supplement Table 1.</b> The expression of genes in morning and evening<br> <b>Abstract</b> <b>Background:</b> This study aimed to investigate the expression of regeneration-related genes in canine urine during bladder repair. <b>Materials &amp; methods:</b> Canine urine samples were collected after partial cystectomy. Regenerative mRNA of hypoxia-inducible factor (HIF), vascular endothelial growth factor (VEGF), key stem cell transcription factors, and cholinergic signals were detected. <b>Results:</b> HIF-1α, VEGF, CD44, interleukin-6, and prominin 1 expression in canine urine after partial cystectomy exhibited two similar peaks at ~2 weeks. HIF-1α and VEGF expression was higher in the afternoon than in the morning. The expression of key stem cell transcription factors and cholinergic signals also exhibited a rhythm along with bladder healing. <b>Conclusions:</b> The expression of HIF-1α, VEGF, key stem cell transcription factors, and cholinergic signals exhibited a time curve distribution during canine bladder healing. The expression trend of some regenerative genes was similar during bladder healing, and a cooperative effect may exist.