Expression data from Hoxb13+ Prostate Luminal Epithelial Cells

A subpopulation of prostate luminal epithelial cells has been previously reported to be sufficient to regenerate prostatic architecture following consecutive rounds of androgen deprivation/repletion. This functional characteristic suggest prostate luminal epithelial cells as the putative cell-of-origin for castration-resistant prostate cancer - which more notizable fenotype is the lack of response to androgen deprivation thereapy. We used microarrays to profile the androgen-induced transcripts in luminal prostate epithelial cells involved in a cycle of prostate regression/regeneration in Hoxb13-rtTA|TetO-H2BGFP transgenic mice. Twelve-week-old male Hoxb13-GFP mice carrying the Hoxb13-rtTA transgene and a Tetracycline operator–Histone 2B-Green Fluorescent Protein (TetO-H2BGFP), which results in GFP expression restricted to luminal epithelial Hoxb13+ cells, were castrated via bilateral orchiectomy. A cycle of prostate regression/regeneration was induced by allowing murine prostates to regress for six weeks to reach the fully involuted state. Following euthanization, prostate luminal epithelial cells were FACS sorted for RNA extraction and hybridization on ClariomD (MTA-1.0) Affymetrix microarrays. We sought to obtain homogeneous populations of luminal epithelial cells at each treatment group (untreated, n=3; ADT, n=3; and ADT+TR, n=4) in order to increase the resolution of expression profiles. To that end, we isolated luminal epithelial cells based on their expression of the GFP fluorescent protein, their positive expression of CD24, intermediate expression of CD49f, and negative expression of the immune markers CD45, F4/80, and CD11b cells. Biological replicates of each treatment group (n≥3), were compared.