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Ribosome profiling of mitotic exponential cells expressing either Dbp1 or Ded1, at 30C or 37C

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NIAID Data Ecosystem2026-05-02 收录
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We replaced the DED1 ORF with DBP1 and tested vegetative growth at 30C or 37C to determine helicase specificity and temperature-dependent effects. We found that DBP1 expressing cells do not downregulate all of the housekeeping genes that DED1 expressing cells normally down regulate at high temperature Overall design: We replaced the DED1 ORF with DBP1 and tested vegetative growth at 30C or 37C to determine helicase specificity and temperature-dependent effects

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2025-01-30
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