Analysis of the chromatin accessibility in CPSF6-RARG or NUP98-RARG overexpressing hCD34+ cells compared to control hCD34+ cells using ATAC-seq.

We found that RARG fusions selectively upregulated BCL2 and ATF3 in HSPCs, driving uncontrolled proliferation and disrupting the differentiation of RARG-AML cells.To investigate the mechanism how RARG fusions mediate activation of their downstream targets such as BCL2 and ATF3, we defined the chromatin accessibility using ATAC-seq assay in human CD34+ (hCD34+) cells with or without CPSF6::RARG or NUP98::RARG overexpression. 10,0000 hCD34+ cells with or without CPSF6::RARG or NUP98::RARG overexpression were harvested and performed the ATAC-seq assay according to the manufacturer’s protocol using the Hyperactive ATAC-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China, #TD711).