Effect of LLC1 tumor cell conditioned medium on RNA expression in mouse osteoblasts (MC3T3 cells). Effect of LLC1 tumor cell conditioned medium on RNA expression in mouse osteoblasts (MC3T3 cells)

MC3T3 osteoblasts were grown in culture medium in the presence of 10 mM beta-glycerophosphate and and 100 micro g per mL of ascorbic acid and 25% LLC1 conditioned medium or vehicle for 7 days. Cells were lysed an RNA was isolated. Overall design: The goal of the experiment was to determine the effect of LLC1 conditioned medium on MC3T3 cell RNA expression . To this end, MC3T3 cells will grown in medium supporting mineralization. Cells which treated with either LLC1 conditioned medium or vehicle for 7 days. Cells were collected, lysed an RNA was obtained for generation of libraries.