Full-length transcriptome reconstruction reveals large diversity of RNA isoforms and open reading frames in rat hippocampus

We developed a hybrid-sequencing workflow, combining next-generation and third-generation sequencing, to reconstruct full-length transcriptomes. Integrating with polysome profiling and ribosome footprinting data, we predicted isoform–specific translational status and reconstructed ORFeome. Moreover, we identified isoforms with specific subcellular localization pattern in neurons. At least two replicates were performed on the adult rat hippocampus.