Organic Carbon Plays an Important Role in Antarctic Lake Region Soil Bacterial Diversity
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16S rRNA gene amplicons were sequenced on an Illumina MiSeq platform, and 250-bp paired-end reads were generated. Clean reads were obtained by removing barcode and primer sequences, trimming end bases and filtering low-quality bases, with QIIME (version 1.7.0) (Caporaso et al. 2010b), chimeras were detected by the UCHIME algorithm (Edgar Robert C. et al. 2011) and against the Gold database, and finally the chimeric sequences and lllumina adapters were removed
创建时间:
2018-12-04



