Supplementary Figure 23 Helium ion microscopy (HIM) buffer and non-phosphorylated controls preclude salt precipitation

Helium ion microscopy (HIM)
buffer and non-phosphorylated controls preclude salt precipitation. In order to determine that our proteins were forming fractal-like
patterns and it was not salt inducing the patterns, a buffer and
non-phosphorylated proteins sample controls were used to preclude salt
precipitation. (A) Usual HIM square
salt crystals on a glass surface. (B)
Deposited HNG buffer (50 mM Hepes, 100 mM NaCl, 5% glycerol, pH.7.4, buffer
proteins are stored in) on silicon wafer shows no structures on the surface. (C) 3 µM non-pY-AtzAM1 and 2 µM AtzCM1
control shows no fractal-like structures. (D)
3 µM non-pY-AtzAM1 and 1 µM AtzCM1 show no fractal-like structures. All
controls demonstrate that fractal structures are formed by phosphorylated
protein components.