scRNA-seg analysis of E12.5, E14.5, E15.5, and E18.5 mouse whole palate

Whole palatal tissues from E12.5, E14.5, E15.5, and E18.5 embryos were digested and dissociated using TrypLE Express enzyme (Thermo Fisher Scientific, 12605010) at 37°C and 600 rpm for 15-30 minutes in a thermomixer (Thermo Fisher Scientific, 2231000269). The single-cell suspension was processed into a single-cell library using 10x Genomics Chromium single-cell 3’ v3 reagent kits (10x Genomics, PN-1000269) according to the manufacturer’s protocol. Sequencing was performed on a NovaSeq 6000 (Illumina), and the quality control, sequence alignment, and read counts were evaluated using Cell Ranger 4.0.0.