Embryo BAG neurons tiling array

modENCODE_submission_2499 This submission comes from a modENCODE project of Robert Waterston. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Our experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: Transcript tiling array analysis EXPERIMENT TYPE: Transcript tiling array analysis. BIOLOGICAL SOURCE: Strain: MT17370 (engineered, target gene gcy-33 tagged by GFP); Tissue: BAG neurons (embryonic); Developmental Stage: Mixed stage of embryos 20dC; Genotype: nIs242[gcy-33::GFP];lin-15(n765); Sex: Hermaphrodite; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Strain MT17370 (engineered, target gene gcy-33 tagged by GFP); temperature 25; Developmental Stage Mixed stage of embryos 20dC; Tissue BAG neurons (embryonic)