Fucosyltransferase 8-derived circular RNA drives M2 polarization of macrophages through ENO1-TNF signaling axis to promote lung cancer progression

CircFUT8 (hsa_circ_0003028) was significantly upregulated in lung cancer tissues, correlating with advanced stages and poor prognosis. It enhanced lung cancer cell proliferation, migration, and invasion while inhibiting apoptosis in cellular and animal models. Mechanistically, circFUT8 directly binds ENO1 to form an RNA-protein complex, promoting M2 macrophage polarization. Silencing circFUT8 reversed these effects by suppressing ENO1 and M2 polarization, inhibiting tumor progression. Overall design: To further explore the downstream pathways and functions regulated by circFUT8, we used RNA-seq to detect the functional enrichment and pathway changes of downstream genes regulated by circFUT8 silencing in A549 and H1299 cells.