Gene expression of wild-type C57BL/6 mouse lungs infected with C2-202 human metapneumovirus (HMPV)

Human metapneumovirus (HMPV) is a primary causative agent of acute lower respiratory tract infections. We used single cell RNA-sequencing (scRNA-seq) to assess lung immune profiles in a mouse model of HMPV infection. Mice were either infected with C2-202 HMPV (5E5 total PFU) or mock-infected with cell lysate (resuspended in PBS to total 100-uL volume and delivered intratracheally). Cells were collected from lungs of mice euthanized day 1 post-infection, processed to single cell suspensions, and analyzed using scRNA-seq.