Enterobacter cloacae strain:AT070Pip076 | isolate:AT070Pip076 Genome sequencing and assembly

Sepsis is a severe disease treated with high doses of antibiotics. This treatment does not only eliminate pathogenic bacteria but parts of the microbiome community as well leading to a disbalance. However, some bacteria are able to survive such treatment due to certain survival and resistance mechanisms. Not only antibiotic resistance mechanisms but also forming strong communities via biofilm formation promotes cell survival. Here, we investigated the properties of the isolate AT70PIP076 from a sepsis patient treated with Piperacillin and Tazobactam. After biochemical analysis and and Maldi-TOF analysis the strain was found to be Enterobacter cloacae. In addition to in vitro antimicrobial susceptibility testing the genome was further investigated in situ in regard to antibiotic resistance. Further live/dead staining and the biofilm formation was investigated using confocal laser microscopy (CLSM). The genome shows presence of EU554560, bcsABZC_AP010953, ehaB, KF662843 and crl. The understanding of the underlying mechanism of biofilm formation in pathogen survival under antibiotic treatment might contribute to the discovery of potential targets to treat sepsis in the future.