Complementation of ΔFghtf1 and ΔFvhtf1 mutant by Fusarium Htf1 homologous genes.

(A) We complemented F. graminearum gene deletion mutant (ΔFghtf1) with F. verticillioides FvHTF1 (ΔFghtf1-Fv) and F. oxysporum FoHTF1 (ΔFghtf1-Fo). ΔFght1-Fv and ΔFght1-Fo produced abundant macroconidia borne on terminal phialides in contrast to ΔFghtf1 which produced aberrant terminal phialides and failed to form macroconidia and. Bar = 20 µm. (B) In F. verticillioides gene deletion mutant (ΔFvht1), microconidial chains are absent instead microconidia assemble in abnormal false heads (a–c). F. graminearum FgHTF1 complemented strain (ΔFvht1-Fg) showed protypical chains and false heads of microconidia found in wild-type F. verticillioides. Bar = 50 µm.