ATR/CHK1/WEE1 dependency in SRSF2-mutated myeloid malignancies

The aim of this experiment is to characterize mRNA splicing in clones derived from the K562 cell line, which have been genetically engineered using CRISPR/Cas9 to carry SRSF2 mutations frequently observed in myeloid malignancies SRSF2 specific guide RNAs which harbored substitution mutations were used to target the nucleotides that code for Proline at amino acid position 95 (P95). Mutations that replace amino acid Proline (P) at position 95 (P95) on SRSF2 were introduced individually in different K562 clones. Proline was substituted with arginine (P95R), leucine (P95L) or histidine(P95H). Subsequently, RNA-sequencing was carried out from individual clones.