MutWP3__CRUK_Grand_Challenge_Mutographs_of_cancer___lifestyle

Studies of the links between obesity and cancer in human patients are confounded by several factors including genetic susceptibility to high body mass index (BMI), high meat consumption with concomitant exposure to food-borne carcinogens, and high caloric intake. Carcinomas will be sequenced from genetically susceptible F1 backcross mice with high body mass index (BMI): males are particularly susceptible to squamous carcinoma formation even on a normal diet. Skin, colon or pancreatic cancers are also available from mice, rats or hamsters that have undergone caloric restriction, or were fed a high fat diet. Whole genome sequencing of this wide range of tumour types from defined model systems will identify any effects of non-mutagenic agents in shaping the architecture of tumour genomes, for comparison with specific signatures found in subsets of human cancers. The samples include: A: DNA and RNA from mouse tumours from the FVBx Mus Spretus backcross population that have a range of BMI values taken at age 8 weeks. Tumours were induced by sequential DMBA/TPA treatment to generate squamous carcinomas B: DNA and RNA from DMBA/TPA squamous carcinomas from mice that were either fed a high fat diet or were maintained under caloric restriction.