Male autism spectrum disorder is linked to brain aromatase disruption by prenatal BPA in multimodal investigations and 10HDA ameliorates the related mouse phenotype (BPA)

Pregannt Cyp19-EGFP mice (maintained on a soybean free mouse chow diet) were treated with either 50μg/kg/day Bsiphenol A or vehicle (ethinol in peanut oil) between gestational days (GD) 10.5-14.5. On GD15.5, fetuses were dissected and sex determined by SRY genotyping. Fetuses were obtained from different dams. To investigate the effect of prenatal BPA expsoure on offspring RNA expression, Cyp19-EGFP mice dams were treated with either 50μg/kg BPA or vehicle (peanut oil) at GD10.5-GD14.5. The cortex of the brain was rapidly dissected from the fetuses at GD15.5.