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Effects of different inhibitors on NMDA-induced MARK/Par1 activation.

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https://figshare.com/articles/dataset/_Effects_of_different_inhibitors_on_NMDA_induced_MARK_Par1_activation_/1400983
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Hippocampal neurons were pretreated with CNQX and TTX for 30 minutes, then stimulated with 50 μM of NMDA for 5 min (NMDA stimulation). For treatment of different inhibitors (NMDA + inhibitor), the indicated inhibitor was included in the pretreatment and during NMDA stimulation. Neurons were lysed and immunoblotted for phospho-Par1, Par1 and tubulin. Blots were quantified by measuring the phospho-Par1 band intensity and normalizing to the intensity of the Par1 bands.
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