Hans Ris (2010) CIL:7504, gallus gallus, erythrocyte. CIL. Dataset

A drop of a preparation of chicken erythrocyte nuclei was exposed to low ionic strength and centrifuged onto a formvar-carbon film, fixed with paraformaldehyde and negatively stained with sodium phosphotungstate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 45 degrees. A grouped image of the same field at a 55 degree tilt provides an oblique stereo view of the tangle of chromatin fibers in the dispersed nucleus.

一滴鸡红细胞核的制备液经低离子强度处理后，被离心沉积于形式碳膜上，经多聚甲醛固定，并使用磷酸钨酸钠进行负染色。图像在威斯康星大学HVEM设施中，以40KX的放大倍数和1MeV的加速电压进行记录。本图像是在45度倾斜的样品角度下拍摄的。同一视野的55度倾斜组合图像提供了散布核中染色体纤维错综复杂的斜视立体视图。