Additional file 1 of Transcriptional analysis reveals a markedly reduced expression of the voltage-dependent calcium channel α2δ1 subunit in canine prostate cancer compared to benign prostatic hyperplasia

Additional File 1. Supplementary Figure 1. Scatter plot showing RNA integrity number and RNA concentration of the samples used in NanoString profiling. Supplementary Figure 2. Volcano plot showing 2923 DEGs in PC vs. BPH (-1>log2FC>1, FDR>0.05) out of 12185 identified genes. After additional filtering (mean normalized CPM >10 in each group), DEGs in green were selected for validation by targeted mRNA profiling (NanoString technology) based on the highest differences in expression and presence of mRNA in both samples (PC and BPH). For clarity, an enlarged section of the volcano plot shows the DEGs upregulated in PC. Supplementary Figure 3. A) Full-length blot presented in Fig.3A, and B) corresponding Stain-Free loading control. C) Blot replicate used together with A) for densitometric analysis presented in Fig. 3B. and D) corresponding Stain-Free loading control.