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CaltechDATA2025-11-09 更新2026-04-16 收录
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https://data.caltech.edu/doi/10.22002/5j6gh-dj551
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Expi293F cells were DNA transfected with NYESO1/D9/EABR or NYESO1/D9 pMHCI constructs, and supernatant was concentrated using 100 kDa MWCO (EMD Millipore; #UFC910096) to be ultracentrifuged on a 20% w/v sucrose cushion before SEC purification. Elution volumes corresponding with extracellular vesicles (Fig. 1D) were collected, digested with trypsin, and run on an Orbitrap Eclipse Tribrid Mass Spectrometer. Proteins were searched and identified against the human proteome using Proteome Discoverer 3.2.
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California Institute of Technology
创建时间:
2025-11-09
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