Figure_6 .jpg

Measurement of the perinuclear localization of ZOL and the positive control for macropinocytosis, dextran, in the absence and presence of 30 microM Dynasore. Violin plots display the HGF perinuclear proportion of intracellular TRITC-dextran (A) and AF647-ZOL (D) fluorescence. Horizontal red lines indicate the average perinuclear proportion of intracellular fluorescence from all cells analyzed per condition via CellProfiler™ software. The triple asterisks *** indicate p equal to or less than 0.001. Representative 63X oil immersion objective photomicrographs of TRITC-dextran intracellular distribution in the absence (B) and presence (C) of Dynasore. Representative 63X oil immersion objective photomicrographs of AF647-ZOL intracellular distribution in the absence (E) and presence (F) of Dynasore. Scale bar = 20 microm.