RNAseq-Fragaria ananassa-JWM

RNA-Seq analysis of octoploid strawberry (Fragaria × ananassa) Total RNA was isolated from the fruits of red and white strawberry varieties at T and R stages using an RNA isolation kit (Waryong, Beijing, China). After the RNA extract had been treated with DNase I, first-strand cDNA was synthesized with a RevertAid First-StrandcDNA synthesis kit (Thermo Scientific Inc., MA, USA). A total of 3 μg RNA per sample was used as input material for the RNA sample preparation at T and R stages from the white and red strawberry varieties. Sequencing libraries were generated using NEBNext® Ultra™ RNA LibraryPrep Kit for Illumina® (New England Biolabs, MA, USA) according to manufacturer’s manual. The libraries were sequenced using Illumina HiSeqTM 2000 (Illumina incorporation, San Diego, California, USA).