Supplemental Material for Pujari and Cullen, 2024

Signaling pathways control the response to stimuli. In yeast, a signaling (MAPK) pathway controls a fungal behavioral response called filamentous growth. A genetic screen was performed to identify spontaneous mutants that show hyperactivity of a MAPK pathway-dependent reporter. Select mutants were analyzed by whole-genome sequencing. New alleles in known regulatory proteins were identified, including an inhibitory domain in the C-terminus of the transcription factor Ste12p. Our results highlight the combinatorial effects of multiple positive and negative regulatory inputs in regulating a MAPK-dependent differentiation response.

信号通路调控对刺激的反应。在酵母中，一条信号（MAPK）通路控制一种称为丝状生长的真菌行为反应。通过遗传筛选，识别出表现出MAPK通路依赖性报告基因超活性的自发突变体。经过全基因组测序分析了部分突变体。在已知调控蛋白中发现了新的等位基因，包括转录因子Ste12p的C端抑制结构域。我们的研究突出了在调控MAPK依赖性分化反应中，多种正负调控输入的组合效应。