METHOD FOR DESIGNING RNA BINDING PROTEIN UTILIZING PPR MOTIF， AND USE THEREOF
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
下载链接：
https://www.forestdata.cn/dataDetail.html?id=2c8137b7-d2ad-433c-90fd-1712db65c67d
下载链接
链接失效反馈官方服务：
资源简介：
A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an a-helix structure， and is represented by the formula 2， wherein， in the formula 2， Ai to A12 independently represent an amino acid; X does not exist， or is a moiety of 1- to 9 amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an a-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3， wherein， in the formula 3， the amino acids are numbered "i" (-1)， "ii" (-2)， and so on from the C-terminus side， provided that Liii to Lvii may not exist)， and combination of three amino acids Ai， A4 and Li， or combination of two amino acids A4， and Li is a combination corresponding to a target RNA base or base sequence.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
