The sulfur stress response caused by sodium hydrosulfide, allicin and diallyl polysulfanes in Bacillus subtilis as revealed by transcriptomics

In this study, we compared the transcriptome signatures in response to sodium hydrosulfide (NaHS), allicin and diallyl tetrasulfane (DAS4) exposure in the Gram-positive bacterium Bacillus subtilis. This analysis revealed that NaHS, allicin and DAS4 caused a similar thiol-specific oxidative and electrophile stress response as well as protein and DNA damage. B. subtilis wild-type cells were grown in Belitsky minimal medium (BMM) to an OD500 of 0.4 and harvested before and 30 minutes after treatment with 2.5 mM NaHS, 90 µM allicin or 92 µM DAS4. Microarray hybridizations were performed with RNA from two biological replicates.